Shuming wang citrus gene editing
WebDec 9, 2016 · Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. WebMore recently, CRISPR/Cas9 mediated editing of citrus Nonexpressor of Pathogenesis-Related 3 (CsNPR3) gene, resulted in induced biotic stress tolerance in sweet orange cultivar 'N7-3 ′ (Mahmoud ...
Shuming wang citrus gene editing
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WebAug 1, 2024 · Engineering of citrus to obtain huanglongbing resistance. C Las cultures on hairy roots enable investigations in real C Las/citrus pathosystem. New C Las-resistant genotypes, sexually compatible with Citrus, have been reported. Putative target genes that may be involved in bacterial infection have been reported. WebHigh concentrations of contaminants such as ammonia nitrogen and organic matter in full-strength wastewater severely inhibit the growth of microalgae, contributing to lower biomass accumulation and contaminant removal efficiency. To overcome this limitation, modified biochars prepared from pine sawdust and sugarcane bagasse were used in this study as …
WebOct 23, 2024 · 2.2. Phase II: Genetic Transformation for Stress Tolerance (2000–2013) To develop stress-tolerant citrus for both biotic and abiotic stresses, Agrobacterium-mediated transformation has been the most widely used gene transfer method in citrus.In order to obtain a high degree of genetic transformation precision, different types of citrus explants … WebDisclosed herein are methods and materials for editing genes in citrus cells. Specifically exemplified is the implementation of an optimized Cas9 and the CRISPR type II class nuclease. Also exemplified is the use of a U6-1 promoter for driving expressing of editing constructs in citrus cells. Various protocols and sequences for editing genes are …
WebsgRNA system—a system that holds significant promise for the study of citrus gene function and for targeted genetic modification. Citation: Jia H, Wang N (2014) Targeted Genome Editing of Sweet ... WebOct 20, 2024 · Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. 126 . Nature Biotechnology. 36, 950-953. 127 . 128 . Figure legend . 129 . Figure 1. Highly efficient base editors for citrus gene editing. (a) Illustration of ABE8e adenine . 130 . base editing (ABE) system for citrus. CmYLCV, Cestrum yellow leaf curling virus ...
WebApr 7, 2014 · Given that Cas9/sgRNA works in transgenic citrus, Cas9/sgRNA-mediated citrus genome editing holds significant promise for studies of citrus gene function and for targeted genetic modification. In conclusion, Cas9/sgRNA appears to be a potentially valuable tool for creating new citrus cultivars with beneficial traits for both growers and …
WebSep 1, 2024 · Plant Cell Rep (2024) 36:1883–1887 DOI 10.1007/s00299-017-2202-4 FOCUS ARTICLE Rapid and efficient CRISPR/Cas9 gene editing in Citrus using the YAO promoter 1 1 1,2 1 • • • Fei Zhang Chantal LeBlanc Vivian F. Irish Yannick Jacob Received: 3 July 2024 / Accepted: 23 August 2024 / Published online: 1 September 2024 Springer-Verlag GmbH … birmingham 2022 tickets log inbirmingham 2022 ticket pricesWebOct 24, 2024 · In a complementary approach, Nian Wang has been using a genome-editing technology, the CRISPR/Cas system, to delete and modify citrus DNA sequences in order to change the citrus proteins that are targeted by the bacterial effectors, ... the CRISPR/Cas system is being used to modify citrus genes targeted by CLas effectors, ... birmingham 2022 resale platformWebCRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be cre … birmingham 2022 triathlon courseWebJun 22, 2024 · Genome editing offers promising solutions to genetic disorders by editing DNA sequences or modulating gene expression. The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) technology can be used to edit single or multiple genes in a wide variety of cell types and organisms in vitro and in … dancing with the stars week 3 scoresWebAug 5, 2024 · We anticipate that future precision genome-editing efforts aimed at the CsACD2 promoter or coding sequences, via CRISPR technology (Jia et al., 2024; Wang 2024), to fine tune CsACD2 gene expression or interaction with SDE15 without causing spontaneous plant cell death could provide new possibilities to generate citrus resistance … dancing with the stars week 3WebMar 18, 2024 · The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpress multiple single guide RNAs (sgRNAs) to achieve multiplex gene editing in plant cells. One is to construct … dancing with the stars week 5 results