Hilic-spe
WebHyperSep™ Tip Microscale SPE Extraction Tips, 10-200µL Catalog number: 60109-209 Related applications: Chromatography Technical Support Customer Service Get faster … WebiSPE-HILIC cartridges are designed for the purification of polar and hydrophilic compounds using Hydrophilic Interaction Liquid Chromatography (HILIC). The packed stationary …
Hilic-spe
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WebDec 1, 2016 · Although HILIC SPE is efficient (reproducible and sensitive) for glycopeptide enrichment from mixtures, hydrophilic non-glycosylated peptides are also frequently co-enriched. This represents a particular problem for analysing complex samples as the co-enriched hydrophilic peptides can cause glycopeptide ion-suppression. WebJun 1, 2014 · HILIC-SPE based on Click XIon sorbents was developed for the extraction of triptorelin from plasma samples. Compared to reversed-phase C18 and HLB SPE, this …
WebHILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest. WebJan 18, 2014 · A HILIC SPE column can be employed to trap hydrophilic compounds, but as previously discussed, the analytes must typically be loaded in a solvent with a substantial percentage of an organic modifier. In an on-line HILIC SPE–RP LC system, the analytes must be transported to the RP LC column without excessive organic solvent to ensure optimal ...
WebHILIC SPE can be used for glyco-profiling, i.e., for determining the glycan heterogeneity at one specific glycosylation site, for enrichment of glycopeptides from a complex mixture of peptides, as well as for pre-fractionation of complex samples at the protein or peptide level. In this chapter we present a straightforward HILIC SPE enrichment ... WebHILIC provides a versatile tool for enrichment of glycopeptides before mass spectrometric (MS) analysis, particularly when used for solid phase extraction (SPE), or in combination …
WebDec 24, 2015 · Zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) solid-phase extraction (SPE) combined with direct-infusion nanoESI mass spectrometry (MS) and tandem MS/MS is a well-suited method for the analysis of protein N-glycosylation.A site-specific characterization of N-glycopeptides is achieved by the combination of proteolytic …
WebFeb 20, 2024 · Two groups have separately reported effective methods for the purification of APTS-labeled glycans. Ruhaak et al. demonstrated that hydrophilic interaction … city bus ride sound effects on amazonWebThermo Fisher Scientific dick\u0027s sporting goods in scottsdale azWebApr 14, 2024 · Excess reagents were removed from the samples using hydrophilic interaction liquid chromatography–solid phase extraction (HILIC-SPE) as described in . … citybus ried im innkreis fahrplanWebApr 4, 2014 · Glycosylation represents one of the most complex and widespread post-translational modifications of human proteins. N-linked … dick\u0027s sporting goods in shreveport laWebIn contrast, HILIC (hydrophilic interaction liquid chromatography) is an attractive chromatographic technique for the analysis of these glycoconjugates. Applications usually involve detection by mass spectrometry (MS) to benefit from its high sensitivity, accuracy and resolution as well as its high throughput potential. city bus rideWebDec 30, 2013 · The extraction solution could be directly applied to the HILIC SPE without the solvent evaporation step, which further simplified the analysis processes. And CKs was enriched and purified with one-step pretreatment procedure that significantly improved the analysis efficiency. Subsequently, CKs were separated by RPLC, orthogonal to the HILIC ... citybus route 50WebMar 20, 2015 · The SPE step is also performed in HILIC mode and is compatible with protein precipitation using acetonitrile (ACN) with 0.1% formic acid (FA), allowing direct analysis of the supernatant with... city bus rote 80