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Samples of Camellia sinensiscv. Shucazao (Variety Approval number: CHN20022008), were obtained from the experimental tea garden of Anhui Agricultural University in Hefei, China (north latitude 31.86, east longitude 117.27, altitude 20 m above mean sea level). Leaves were collected at five different stages (bud, 1st … See more The CsF3′5′H gene from the NCBI database was subjected to standard end-to-end PCR reactions, with the primers designed according … See more Total RNA was isolated from Camellia sinensisorgans with RNAiso Plus (Takara, DaLian, China) and RNAiso-mate for Plant Tissue (Takara, DaLian, China), according to the manufacturers’ … See more Protein synthesis was indiced in the yeast culture by the addition of galactose and the microsomal yeast fraction was prepared with MgCl2 as described by Olsen et al. [11]. Protein quantities were estimated according … See more The Gateway® Cloning System was used to construct the vectors provided by Prof Xiang [39] of the University of Science and Technology of China. CsF3′5′H PCR products were obtained by end-to-end PCR and ligated into … See more WebHub teeth are fully crowned to provide for a larger contact area and lower stress under misaligned conditions. Crowned tooth design avoids end loading that occurs on straight … frenchs onion from scratch
Transcriptomic analysis reveals anthocyanin biosynthesis regulation …
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